A STUDY ON THE ROLE OF ZWITTERIONIC PHOSPHATIDYLCHOLINE LIPID (S) IN PHYSICOCHEMICAL FEATURES OF LIPOSOME ENCAPSULATED PACLITAXEL AS A DRUG DELIVERY SYSTEM
Abdulsalam
Kassem
1Department of Molecular Physical Pharmaceutics, Institute of Biomedical Sciences, Tokushima University, 1–78–1 Sho-machi, Tokushima 770–8505, Japan.
author
text
article
2021
eng
The lipid composition of the liposomal membrane plays a crucial role in the physical and pharmacological behaviors of liposomal payloads, and it can be tailored to achieve the desired delivery needs. Thus, the aim of the current study was to develop and optimize paclitaxel (PTX) loaded liposome nanocarrier using a thin-film rehydrating method and studying the influence of different zwitterionic Phosphatidylcholine (PC) lipids with different acyl chains, on the physicochemical properties of the developed PTX liposomal formulations. The studied PC were dipalmitoyl (DPPC), dimyristoyl (DMPC) and palmitoyl-oleoyl (POPC) PCs, in combination with cholesterol. Several characteristics were monitored, including PTX entrapment efficiency (EE) of liposomes, particle size (PS), polydispersity index (PDI), zeta potential (ZP), PTX release in phosphate buffered saline (PBS) with 0.1 tween® 80 and in PBS / 10 % fetal bovine serum (FBS) cell culture medium, as well as in vitro antitumoral and therapeutic effects. The results demonstrated that DPPC exhibited superiority in the physicochemical properties when compared with DMPC and POPC. The in vitro cytotoxicity findings indicate that the PTX-liposome would be a promising substitutional approach for Taxol® as a commercial product with a toxic solvent. The results outcomes indicated that the developed PTX-liposome would be a viable drug delivery system with sustaining effect for management of colorectal cancer cells and further clinical use.
Al-Azhar Journal of Pharmaceutical Sciences
Al-Azhar University, Faculty of Pharmacy
1110-1644
63
v.
1
no.
2021
1
16
https://ajps.journals.ekb.eg/article_153554_7e1a874a736bf01b9355871fbf2ed579.pdf
dx.doi.org/10.21608/ajps.2021.153554
PREPARATION AND CHARACTERIZATION OF (−)-EPIGALLOCATECHIN GALLATE LIPID BASED NANOPARTICLES FOR ENHANCING AVAILABILITY AND PHYSICAL PROPERTIES
Sabry
Eltawela
Department of pharmaceutics and industrial pharmacy, Zagzig university, Egypt
author
text
article
2021
eng
(−)-Epigallocatechin gallate (EGCG) is the most abundant and biologically active polyphenol found in green tea. (−)-Epigallocatechin gallate (EGCG) is highly potent antioxidant therefore, it has great therapeutic effect against cancer disease. Furthermore, it has antibacterial and anti-inflammatory effect. but it has some limitations, such as the poor permeability, the highly affinity to intestinal P-glycoprotein efflux mechanism and short half-life. Therefore, it has very low oral bioavailability. In current study, Precirol® ATO 5 (P), glyceryl monostearate (GMS) and Compritol® 888 ATO (C) were selected as solid lipids and miglyol (M) and sesame oil (SO) as liquid lipids, to develop EGCG-NLC (nanostructured lipid carrier) and EGCG-SLN (solid lipid nanoparticles). EGCG was successfully encapsulated into lipid nanoparticles to improve its bioavailability. EGCG-NLC and EGCG-SLN were formulated using a hot homogenization-ultrasonication technique, and the physicochemical properties were characterized. The developed EGCG-NLCs and EGCG-SLNs showed small and homogeneous particle size approximately (308 and 379 nm) with entrapment efficiency around (76 and 49%), respectively. Also, the resulted formulation EGCG-NLC3 appeared under transmission electron microscope in almost spherical shape.
Al-Azhar Journal of Pharmaceutical Sciences
Al-Azhar University, Faculty of Pharmacy
1110-1644
63
v.
1
no.
2021
17
36
https://ajps.journals.ekb.eg/article_153558_a04f6c77682b2d929bc081b2b842ae54.pdf
dx.doi.org/10.21608/ajps.2021.153558
CYTOTOXIC POTENTIALS AND PHYTOCONSTITUENTS PROFILING OF BLEPHARIS EDULIS (FORSSK.) PERS. USING UHPLC/Q-TOF-MS-MS
Taha
Abdel Maboud
Department of Pharmacognosy, Faculty of Pharmacy, Al-Azhar University, Cairo, Egypt.
author
text
article
2021
eng
Recently, the demand for discovery of safe anticancer drugs from nature has been extensively increased to avoid cancer chemotherapy side effects. Blepharis edulis (Forssk.) pers (Acanthaceae) is a perennial herb growing in Egypt with many traditional and pharmacological activities. The in vitro assay as well as identification of phytoconstituents in plant samples by LC/MS/MS spectrometry has reserve time and efforts for rapid screening and identification of biologically active compounds in plants. Herein, we investigated the cytotoxic activity of different fractions of Blepharis edulis methanolic extract using Sulforhodamine B stain (SRB) against three cancer cell lines; Hepatocellular carcinoma (HepG-2), human colon carcinoma (HCT-116), and human breast adenocarcinoma (MCF-7) cell lines. The results revealed that the n-butanol fraction showed the most potent cytotoxic activity against the three tested cancer cell lines with CC50 values 9.12 ± 0.92, 6.79 ± 0.65 and 4.19 ± 0.51 against MCF-7, HCT-116 and HepG2 cell lines, respectively. Moreover, the high resolution the ultra-high-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UHPLC/Q-TOF-MS-MS) metabolomic analysis for the n-butanol fraction as the most active sample has been achieved for the first time. A total of 30 compound of different classes including flavonoids, aromatic aldehydes, aromatic acids and alkaloids, have been identified from Blepharis edulis.
Al-Azhar Journal of Pharmaceutical Sciences
Al-Azhar University, Faculty of Pharmacy
1110-1644
63
v.
1
no.
2021
37
56
https://ajps.journals.ekb.eg/article_153559_c2582cfc469f11534f114cf7494e4624.pdf
dx.doi.org/10.21608/ajps.2021.153559
A COMPARATIVE STUDY OF THE SAUDI ARABIA FLORA, MEDICINAL PLANTS OF AL- MANDAQ PROVINCE, AL-BAHA REGION.
Mohamed
Abdelhady
Department of Biology, Faculty of Sciences & Arts, Al Mandaq, Al-Baha University
author
text
article
2021
eng
Medicinal and aromatic plants are important sources for the production of many pharmaceutical compounds such as antibiotic drugs. The presented study aims to survey and record the various medicinal plant species in Al-Mandaq Governorate - Al-Baha region - Kingdom of Saudi Arabia with a number of targeted locations such as Mashnaih, Darake and Khleb valleys, Medhas and Darake Dams in 2018 and 2019. 160 species of vascular plants belonging to 52 plant families were surveyed and recorded. The study proved that the Poaceae family is the most contain of the plant species (22 species, 13.7%), followed by the Asteraceae family (19 species, 11.8%), then the Fabaceae family (11 species, 6.8%). According to the life forms, the annual plants represent the highest percentage among the recorded plants (56 species, 35%), followed by the perennial herbs (48 species, 30%), then shrubs (26 species, 16.25%) and the least recorded species are bi-perennials (5 species, 3.12 %), Palms and cactus (one species, 0.6%). The recorded results and statistics represent a database of the floral content in the study area and also provide important information for the recorded plants and their uses in the fields of food, ornamental and biological applications.
Al-Azhar Journal of Pharmaceutical Sciences
Al-Azhar University, Faculty of Pharmacy
1110-1644
63
v.
1
no.
2021
57
71
https://ajps.journals.ekb.eg/article_153560_cb480de0c36a1a84c7be90c23e4ea971.pdf
dx.doi.org/10.21608/ajps.2021.153560
DESIGN, SYNTHESIS, AND BIOLOGICAL TRACING OF 99MTC- DPDQA AS A POTENTIAL MARKER FOR TUMOR IMAGING
Mohamed
El-Zahabi
Department of Pharmaceutical Medicinal Chemistry & Drug Design, Faculty of Pharmacy (Boys), Al-Azhar University, Cairo 11884, Egypt.
author
text
article
2021
eng
Thalidomide is a sedative drug discovered at the end of the 50s, it showed high anticancer activity so attempts were made to synthesize thalidomide analogs that had fewer side effects than the parent compound. DPDQA showed higher anticancer activities than thalidomide. Labeling of a DPDQA with 99mTc using Na2S2O4 as reducing agent was performed. The dependence of the radiolabeling yield on the concentrations of DPDQA and reducing agent, pH of the reaction mixture, reaction time, and reaction temperature was studied. Bio-distribution studies in mice with tumor induced in the right thigh were carried out. The tumor infected thigh/contralateral thigh uptake ratio (T/NT) was evaluated. The time for the maximum accumulation of the 99mTc–DPDQA in the tumor site was evaluated after administration of the compound.
Al-Azhar Journal of Pharmaceutical Sciences
Al-Azhar University, Faculty of Pharmacy
1110-1644
63
v.
1
no.
2021
72
85
https://ajps.journals.ekb.eg/article_153562_4866c1e2854aace242e7c83dded06822.pdf
dx.doi.org/10.21608/ajps.2021.153562
SYNTHESIS AND BIOLOGICAL EVALUATION OF NOVEL PYRAZOLO[3,4-D]PYRIMIDINE DERIVATIVES OF EXPECTED ANTICANCER ACTIVITY
Farag
Sherbiny
Department of Organic Chemistry, Faculty of Pharmacy (Boys), Al-Azhar University, Cairo, Egypt.
author
text
article
2021
eng
A series of novel pyrazolo[3,4-d]pyrimidine derivatives have been designed based on chemical modifications on the general features of the reported and clinically used EGFR-TKIs such as repalcing of quinazoline moiety of reported EGFR-TKIs as erlotinib by pyrazolo[3,4-d]pyrimidine nucleus,introducing different hydrophobic moieties including phenyl, aromatic heterocyclic, fused aromatic or aliphatic structures , introducing different linkers which may be one atom , two atoms, three atoms, four atoms and five atoms and introducing of phenyl ring at position-3 of 1H-pyrazolo[3,4-d]pyrimidine nucleus to occupy the hydrophobic region II of ATP binding site. All the new synthesized compounds were biologically screened in vitro for their cytotoxic activities against four cancer cell lines namely, HepG-2, MCF-7, HCT-116, and Hela. The results of cytotoxic evaluation indicated that compound VI was found to be the most prominent broad-spectrum cytotoxic activity and significantly more potent than doxorubicin with IC50 values of 6.18, 6.48, 4.03, and 5.82μM against tested cell lines. In addition, compounds IXa,b displayed promising cytotoxic effect against all tested cell lines with IC50 values less than 30 μM compared with doxorubicin as a control drug. Besides, compound X possessed excellent anti-proliferative activities against the four cell lines with IC50 values ranging from 18 μM to 39.5 μM. Structural pharmacophoric features indicated that pyrazolo[3,4-d]pyrimidine scaffold having a four atoms linker as thiosemicarbazide moiety as compounds IXa,b which substituted with aliphatic moiety at the 4-position was more potent than those possessing one atom, two atoms, three atoms and five atoms linkers which lead to significant decrease in cytotoxic
Al-Azhar Journal of Pharmaceutical Sciences
Al-Azhar University, Faculty of Pharmacy
1110-1644
63
v.
1
no.
2021
86
100
https://ajps.journals.ekb.eg/article_153563_181d1423cb8409012c16a8c8c88f9853.pdf
dx.doi.org/10.21608/ajps.2021.153563
THE CORRELATION OF THE BRCA-IRIS GENE EXPRESSION AND BIOMARKER GENES IN BREAST CANCER PATIENTS: CORRELATION WITH TUMOR PROGRESSION AND RESPONSE TO TREATMENT
Safaa
Fawzy
Department of Cancer Biology, Virology and Immunology Unit, National Cancer Institute, Cairo University, Egypt.
author
text
article
2021
eng
Background: Triple-negative breast cancer (TNBC) is an aggressive phenotype with bad prognosis and poor survival. The prognostic and predictive values of BRCA1-Iris, octamer-binding transcription factor 4 (oct 4), cyclin D1andsurvivin were assessed in TNBC patients compared with N-TNBC patients. Method: RNA expression levels of BRCA1-IRIS, Oct4, Cyclin D1 and Survivin gene were tested in for 100 breast cancer patients by qRT-PCR. Result: overexpression ofBRCA1-IRIS RNA was found in 49 % of breast cancer patients [(35(71 %) of TNBC cases vs 14(28.6% of N-TNBC] (p < 0.001). There was significantly correlated between the BRCA1-IRIS and tumor stage, ER and PR (p=0.036, p < 0.001 and p=0.006; respectively) in breast cancer patients. In TNBC or N-TNBC, no statistically significance between BRCA1-IRIS and any clinicopathological features of patients. There was significant association between BRCA1-IRIS positive and the expression of oct4, cyclin D1 and survivin gene in TNBC patients (p < 0.001, p=0.001 and p=0.002; respectively). There was a strong significant correlation between the expression of oct4 and the BRCA1-IRIS negative (p < 0.001) in TNBC group. No significant association between BRCA1-IRISpositive in N-TNBC and any of the gene expression. BRCA1-IRISnegative was correlated significantly with oct4 and cyclin D1expression (p=0.001each).No significant correlation between the expression RNA level of oct4, cyclin D1 and survivin gene and the expression RNA level of BRCA1-IRIS except lymphnode with survivin gene expression (p=0.05). No significant relation between the expression level of oct4, cyclin D1 and survivin gene and the relevant clinicopathological features with or without BRCA1-IRIS expression. Expression level of survivin and cyclin D1 was significantly correlation with the response to treatment (p=0.001 and p < 0.001). No significant different between overall survival and BRCA1-IRIS expression (p=0.291 long rang). However, disease-free survival was increased in BRCA1-IRIS positive cases (p = 0.052 long rang)
Al-Azhar Journal of Pharmaceutical Sciences
Al-Azhar University, Faculty of Pharmacy
1110-1644
63
v.
1
no.
2021
101
125
https://ajps.journals.ekb.eg/article_153564_e786f355cc3cd7a702e28fd26344ff18.pdf
dx.doi.org/10.21608/ajps.2021.153564
THE RNA EXPRESSION LEVEL OF SURVIVIN, OCT 4 AND CYCLIN D1 GENES IN TRIPLE NEGATIVE AND NON-TRIPLE NEGATIVE BREAST CANCER
Safaa
Fawzy
Department of Cancer Biology, Virology and Immunology Unit, National Cancer Institute, Cairo University, Egypt.
author
text
article
2021
eng
Background: the purpose of the present study to investigate the RNA expression level of Oct4, Survivin and Cyclin D1 genes in Triple-negative breast cancer (TNBC) and compared to non-triple negative breast cancer (non-TNBC) group. Also, correlate the results of gene expression with clinicopathological features of patients. Method, RNA expression levels of Oct4, Survivin and Cyclin D1 was tested for 100 breast cancer (BC) patients [formalin fixed paraffin embedded tissue (FFPE)] diagnosed as invasive duct carcinoma in Pathology Department, National Cancer Institute (NCI), Cairo University. The qRT-PCR technique is use and the results correlated to clinico-pathological characteristics of patients and survival rates. Result, in (TNBC) patients, oct4, survivin and cyclin D1 showed positive expression in 30 (60%), 32(64%) and 32 (64%) (p < 0.001, p= 0.001 and p= 0.003;respectively). In non-TNBC patients, the positive expression of oct4, survivin and cyclin D1 gene was 11(22%), 15(30%) and 17(34%) (p < 0.001, p= 0.001 and p= 0.003;respectively). All patients in TNBC have negative ER, PR and HER-2 receptor (p=0.001). There was highly statistically significant difference between oct4 gene expression and all the clinico-pathological features except the family history. A significant difference between cyclin D1gene expression and all the clinico-pathological features except the tumor size, tumor grade and lymphnode status. Statistically difference was found between survivin gene expression and all the clinico-pathological features except the menopause state, tumor stage and tumor grade. Triple negative breast cancer patients showed significantly decreased DFS (p < 0.001, log rank) and OS (p= 0.002, log rank) when compared to those with non-TNBC patients. In TNBC group, patients with positive expression of survivin was significantly associated with decreased OS (p= 0.03, log rank). TNBC tumors with positive oct4 and cyclin D1 had reduced OS compared to those negative to oct4 and cyclin D1 but without significant difference (p= 0.8 and p= 0.09, respectively, log rank). In non-TNBC group, with positive oct4, cyclin D1 and survivin did not significantly differ in terms of DFS and OS when compared to those with negative expression. Conclusion: Oct4 and survivin expression gene are better marker used for diagnosis in TNBC and for molecular targeting therapy of TNBC treatment. Cyclin D1 expression used as a marker for aggressive TNBC. The TNBC tumor possibly respond to treatment that downregulates cyclin D1 amplifica tion. More studied and large sample size is needed.
Al-Azhar Journal of Pharmaceutical Sciences
Al-Azhar University, Faculty of Pharmacy
1110-1644
63
v.
1
no.
2021
126
148
https://ajps.journals.ekb.eg/article_153565_746ca6f4997526943c0b1d4cdc561ed3.pdf
dx.doi.org/10.21608/ajps.2021.153565
GENOTYPIC DETECTION OF THE VIRULENCE FACTORS OF UROPATHOGENIC ESCHERICHIA COLI (UPEC) STRAINS ISOLATED FROM PREGNANT FEMALES AND THEIR CORRELATION WITH ANTIBIOTIC RESISTANCE PATTERN
Marwa
Ahmed
1Department of Microbiology and Immunology, Faculty of Pharmacy for Al-Azhar University, Cairo, Egypt.
author
text
article
2021
eng
Urinary Tract Infections (UTIs) during pregnancy are among the most common infections worldwide and can lead to poor perinatal and maternal outcomes, especially in developing countries and requires medical treatment as soon as it is detected. Virulent and resistant strains of Escherichia coli are the most important causative agents for UTIs. The present investigation aimed to detect virulence factors of Uropathogenic Escherichia coli (UPEC), including pap, fim, sfa, aer and hly genes in isolates collected from symptomatic and asymptomatic pregnant females by multiplex polymerase chain reaction (PCR) assay and determine their correlation with antibiotic resistance patterns. Two hundred urine samples collected from pregnant females with or without symptoms of UTI were admitted to antenatal clinic at Obstetrics and Gynecology Department of Al- Zahraa University Hospital in Cairo. Out of 200 cultured urine samples, the cultures that showed ≥ 105 CFU/ml were identified to have significant growth. Found significant bacteriuria was detected in 61 samples and these samples selected for further analysis in this study, the overall prevalence of UTI among pregnant females was (30.5%). Symptomatic pregnant females with UTI were (48.5%) more than asymptomatic (11.3%). The most frequently isolated species was S. saprophyticus (35.0%),followed by E. coli (26.2%),S .aureus (19.4%), C .albicans (5.8%),S. epidermidis (2.9%) and each of K. pneumonia, K .oxytoca, P .vulgaris, E.fecalis, Bacillus spp were(1.9%),while P. auroginosa had the least percentage of isolation (1.0%). Antimicrobial susceptibility pattern was done for 27 UPECisolates showed the highest level of resistance (100%) against β-lactams as (Ampicillin, Penicillin and Ceftriaxone), nitrofurantoin, and ampicillin/sulbactam, while the lowest level of resistance (66.7%) against Cotrimoxazole. Multiple drug resistance (resistance to two or more drugs) was observed in (100%) of the UPEC isolates. The higher level of resistance to antibiotics was observed in symptomatic bacteriuria more than asymptomatic. Among two predominant uropathogens, E. coli showed complete resistance up to 5 different antibiotics of total 11 antibiotics (45.5%) while S. saprophyticus showed complete resistance (18.2%), so E. coli showed higher virulence and resistance than S. saprophyticus to cause UTI, so subjected to Multiplex PCR to detect its virulence factors, which included pap, fim, sfa, aer and hly genes Theadhesive fimH gene was the most frequent in UPEC isolates (92.6%) . These virulence genes detected from total UPEC isolates of symptomatic cases were higher than asymptomatic. On the other hand, UPEC strains carrying the virulence genes were more resistant to the antibiotics used, so these observations confirm the important role of virulence genes of UPEC in in existence of symptoms of UTI and the drug resistance.
Al-Azhar Journal of Pharmaceutical Sciences
Al-Azhar University, Faculty of Pharmacy
1110-1644
63
v.
1
no.
2021
149
172
https://ajps.journals.ekb.eg/article_153566_35ada9c181c26e94f98313fcc9e9002a.pdf
dx.doi.org/10.21608/ajps.2021.153566
MICROBIAL AND PHYSICO-CHEMICAL ASSESSMENT OF WASTEWATER PLANTS BEFORE DISCHARGE IN QARUN LAKE, EL-FAYOUM GOVERNORATE, EGYPT.
Mahmoud
El-sayed
1Department of Botany and Microbiology, Faculty of Science, Al-Azhar University
author
text
article
2021
eng
This study is performed on six wastewater stations that discharging wastewater into Qarun lake, El-Fayoum Govenorate, Egypt, to assessment wastewater treatment on the period from April 2017 to February 2019. Sewage treatment plants use different biological treatment technologies (conventional activated sludge, oxidation ditch, aeration). In this investigation, wastewater samples were collected from the influent and effluent of each plant and transferred directly to central laboratory of Fayoum Wastewater Company for complete analysis. The obtained data reflects a large amount of drainage on the wastewater treatment stations which means high percentages of total coliform and fecal coliform. Also, the high algal content is detected at Tamiya station with total cell count 282-1210/L. Nutrients as nitrate, nitrite, ammonia and phosphate are assessed and exhibit in ranges 4.4-104.5, 0.0-8.0, 1.1-43.0 and 1.0-42.1 mg/L, respectively. On other hand, the increase of TDS and TSS were achieved during summer season. Furthermore, higher values of DO, BOD and COD considered as a strong indicator for higher microbial activity.
Al-Azhar Journal of Pharmaceutical Sciences
Al-Azhar University, Faculty of Pharmacy
1110-1644
63
v.
1
no.
2021
173
193
https://ajps.journals.ekb.eg/article_153567_924643e792e066d7a5625755fee86d78.pdf
dx.doi.org/10.21608/ajps.2021.153567
PREVALENCE AND RELATION OF URINARY TRACT INFECTION BACTERIAL PATHOGENS TO SEX AND AGES AMONG PATIENTS IN THREE ARAB COUNTRIES
Khaled
Azab
1Botany and Microbiology Department, Faculty of Science, Al-Azhar University, Cairo, Egypt
author
text
article
2021
eng
Background: urinary tract infections is one of the most prevalent bacterial infections worldwide. The present study aims to study and survey different isolates from urine specimens from different countries to assess their prevalence and their relation to urinary tract infections. One hundred and sixty-eight samples were randomly collected from three countries, Egypt, Sudan and Saudi Arabia, as follows: 43, 33 and 92, respectively. Samples were collected from private laboratories and from both sexes. The ages of the patients differed between three years and 89 years for females and from one year to 85 years for males between 4 / 2015-7 / 2016. All samples were inoculated on different selective and differential sterile culture media. After growth, isolated bacteria were identified by physiological and biochemical characteristic. Among all clinical samples, five bacterial genera were detected. The isolates were identified as Escherichia coli (93), Klebseilla spp < /strong>. (32), Pseudomonas spp < /strong>. (26), Proteus spp < /strong>. (14), and Staphylococcus spp < /strong>. (3). Regarding the sex of the patient, this study showed that females are more likely to be infected than males, with 53 (31.55%) of the patients being males and 115 (68.45%) of being females. The most common UTI bacterium was Escherichia coli, followed by Klebseilla spp < /strong>. Majority of female infected cases were in menstruation age stage (14-44 years), while majority males were in old age stage (52-85 years). Surveys and studies of infectious factors are considered one of the most important epidemiological tools for tracking infectious diseases and predicting disease patterns, especially with regard to urinary tract infections due to their widespread prevalence and serious complications between the sexes at the global level.
Al-Azhar Journal of Pharmaceutical Sciences
Al-Azhar University, Faculty of Pharmacy
1110-1644
63
v.
1
no.
2021
194
206
https://ajps.journals.ekb.eg/article_153568_0669a9e692740943e1433386312a4c9d.pdf
dx.doi.org/10.21608/ajps.2021.153568
CILOSTAZOL ATTENUATES DIABETIC NEPHROPATHY IN RATS VIA ELEVATION OF PLASMA SOLUBLE RECEPTOR FOR ADVANCED GLYCATION END-PRODUCTS
Soad
Kabil
Department of clinical pharmacology, Faculty of Medicine, Zagazig University
author
text
article
2021
eng
Cilostazol is a phosphodiesterase III inhibitor with antiplatelet and vasodilating properties. Cilostazol effectively attenuated severity of diabetic angiopathy. However, the underlying mechanisms involved in its beneficial effect against diabetic nephropathy are not fully elucidated. Type-1 diabetic rats received cilostazol (25mg/kg/day, oral for 12 weeks). Fasting blood glucose, serum lipids, renal nuclear factor κB (NF-κB) and interluckin-6 (IL-6), kidney function markers, oxidative stress parameters were examined. The plasma soluble receptor for advanced glycation end products (sRAGE) was assayed. Renal advanced glycation end products (AGEs) level was assayed. Renal histopathological study was also performed. The results showed improvement in kidney structure and function post cilostazol treatment. Furthermore, cilostazol elevated the circulating plasma sRAGE level and decreased renal AGEs, NF-κ B and IL-6 levels. Moreover, the renal levels of reduced glutathion, superoxide dismutase and heme oxygenase-1 increased associated with reduction in malondialdehyde suggesting enriched antioxidant status in the kidney. Conclusion, Cilostazol ameliorates diabetic renal injury which may be, in part, due to elevation of circulating plasma sRAGE, lowering of renal AGEs level and in other part the anti-inflammatory action and enhancement of the renal antioxidant status.
Al-Azhar Journal of Pharmaceutical Sciences
Al-Azhar University, Faculty of Pharmacy
1110-1644
63
v.
1
no.
2021
207
223
https://ajps.journals.ekb.eg/article_153569_6996b3d1519e50fbb5a2430554ea2fca.pdf
dx.doi.org/10.21608/ajps.2021.153569