Al-Azhar University, Faculty of PharmacyAl-Azhar Journal of Pharmaceutical Sciences1110-164457120180301STUDY ON THE THERAPEUTIC POTENTIAL OF MELISSA OFFICINALIS (LEMON BALM) ON MALE WISTAR ALBINO RATS WITH INDUCED-HYPOTHYROIDISM1174661810.21608/ajps.2018.46618ENMohamedAbdel-AzizZoology Department, Faculty of Science, (Boys) Al-Azhar University, EgyptJournal Article20190901This study aimed to evaluate the potential improvement of Melissa Officinalis Ethanolic Extract (MEE) against propylthiouracil induced-hypothyroidism. After acclimatization, 50 male Wistar albino rats were randomly divided into five groups; group 1) normal control rats, group 2) normal rats administrated orally with (75 mg/kg) MEE for 60 days, group 3) hypothyroidism-induced rats, group 4) hypothyroidism rats treated orally with Eltroxin<sup>®</sup> drug (100 ug/kg), and group 5) hypothyroidism rats treated with MEE (75 mg/kg). The obtained results revealed that hypothyroidism induction resulted in reduction in the thyroid hormones and affect negatively on the liver and kidney tissues as well as deteriorate their oxidative status. MEE therapy succeeded in amelioration of thyroid function towards normal level without any unfavorable effects on the body physiology as it contains many safe phytochemical compounds. In conclusion, MEE possesses anti-hypothyroidism efficacy and antioxidant properties.https://ajps.journals.ekb.eg/article_46618_a7f906994ea68011a0be695efb5d8d06.pdfAl-Azhar University, Faculty of PharmacyAl-Azhar Journal of Pharmaceutical Sciences1110-164457120180301FORMULATION,CHARACTERIZATION AND IN-VITRO RELEASE OF ORAL FELODIPINE SELF-NANOEMULSIFYING DRUG DELIVERY SYSTEMS18514661910.21608/ajps.2018.46619ENAmrShaabanPharmaceutics and Industrial Pharmacy Department, Faculty of Pharmacy, Al-Azhar University, Nasr City, Cairo, EgyptJournal Article20190901Nanotechnology is a cross-disciplinary field, which involves the ability to design and exploit the unique properties that emerge from man-made materials ranging in size from 1 to greater than 100 nm.A self- nanoemulsifying drug delivery system is a fairly similar liquid lipid dosage form designed for oral delivery which composed of oils, surfactants and possibly cosurfactants or cosolvents.Felodipine is a calcium channel blocker (CCB). It acts primarily on vascular smooth muscle cells by stabilizing voltage-gated L-type calcium channels in their inactive conformation.<br /> The aim of this paper was to formulate Felodipine self nanoemulsifying system to overcome the poor aqueous solubility of Felodipine (3ug/ml) and hence improving its poor dissolution which is the main cause of its poor oral bioavailability. Evaluation, Characterization and In-Vitro release of orally Felodipine Self-nanoemulsifying Drug Delivery Systems were studied in comparison with the market product.<br /> Formulae B18 (composed of 30% Triacetin: 40% Span 80: 30% Transcutol HP) and C19 (composed of 20% Triacetin: 50% Span 80: 30% Ethanol) were selected for Felodipine SNEDDS.Felodipine SNEDDSs prepared using Transcutol HP (B18) exhibited pseudoplastic flow while Felodipine SNEDDSs prepared using ethanol (C19) exhibited Newtonian flow.Formula prepared with Transcutol HP (B18) has smaller droplet size and PDI than that prepared with ethanol (C19). Felodipine formulae showed negative zeta potential values.The in-vitro release can be arranged in descending order as follows: Felodipine formula B 18 > Felodipine formula C 19 > Market product.https://ajps.journals.ekb.eg/article_46619_05db70c19f62f48d73e333fd808c81dc.pdfAl-Azhar University, Faculty of PharmacyAl-Azhar Journal of Pharmaceutical Sciences1110-164457120180301EFFECT OF AGNO3 NANOPARTICLES ON STERILIZATION AND ALKALOID PRODUCTION OF CATHERENTHUS ROSEUS TISSUE BY52614662410.21608/ajps.2018.46624ENHebaShahinGenetic Engineering and Biotechnology Research Institute (GEBRI), Sadat City University, Sadat City, P.O. Box 79/22857, EgyptJournal Article20190901Bacterial contamination is a serious problem in plant tissue culture procedures.<br /> An experiment has been conducted to evaluate the potential of nanosilver (NS) to remove bacterial contaminants from Catherenthus shoot tips and single nodes.<br /> The experiments were performed as a totally randomized design in a faculty with four replications and each replicate with ten explants. Treatments involved NS in two steps (before and after surface sterilization along with control) at three concentrations (25, 50 and 100 mg 1<sup>-1</sup>) at three times (30, 45 and 60 minutes). Plants were grown on MS medium supplemented with 5 mg l<sup>-1</sup>of Kinetin (KIN) and 0.1 mgl<sup>-1</sup> of naphthaline acetic acid (NAA). The results showed that the use of 100 mg l<sup>-1</sup> NS solution after sterilization of the surface resulted in the highest percentage (89%) of disinfected explants. Nano silver solution did not affect the measured characters. According to the data obtained in the experience, it was concluded that NS had a good view to eliminate bacterial diseases in plant tissue culture procedures. Further research on other cultural species requires that in the tissue cultures of other cultures, to enhance the effectiveness of the NS in order to eliminate bacterial diseases.https://ajps.journals.ekb.eg/article_46624_1bebd56afd941458138f9f5bbcc324c9.pdfAl-Azhar University, Faculty of PharmacyAl-Azhar Journal of Pharmaceutical Sciences1110-164457120180301ENHANCED PRODUCTION OF SECONDARY METABOLITES BY METHYL JASMONATE AND SILVER NANOPARTICLES ELICITATION IN TISSUE CULTURE OF CATHARANTHUS ROSEUS (APOCYNACEAE)62694662710.21608/ajps.2018.46627ENHebaShahinPlant Biotechnology Department, Genetic Engineering and Biotechnology Research Institute (GEBRI), Sadat City University, Sadat City, P.O. Box 79/22857, EgyptJournal Article20190901Methyl jasmonate (MeJA) and silver nanoparticles (SNPs) are one of the elicitors that can induce the production of secondary metabolites of <em>Catharanthus roseus</em> L., the two of which incite defense responses.<br /> Methyl jasmonate (MeJA) treatments with different concentrations (0, 10, 50, and 100 µM) were used to enhance high yielding of alkaloid content in <em>Catharanthus</em><em>. roseus</em>. The content of vincristine and vinblastine in the callus of <em>Catharanthus</em><em>. roseus</em> were determined by HPLC.<br /> High content of vinblastine was seen in those explants treated with 75 mg/L SNPs (1.4 µg/g Fresh Weight). The most estimation amount of vinblastine and vincristine were seen in plants treated with 50µM MeJA (1.23 and 0.9 µg/g FW).https://ajps.journals.ekb.eg/article_46627_6e3d472f2ca4044f8caac47e36ae6e86.pdfAl-Azhar University, Faculty of PharmacyAl-Azhar Journal of Pharmaceutical Sciences1110-164457120180301ANTIMICROBIAL & ANTI-DIABETIC EFFECT OF TRITERPENE BARRINGOTOGENOL C GLYCOSIDES ISOLATED FROM AERIAL PART OF MEDICAGOSATIVA L.(ALFALFA) (FAMILY FABACEAE) CULTIVATED IN EGYPT.70884662810.21608/ajps.2018.46628ENMaieMohamedPharmacist at Al-Tawfeek Polyclinic complexJournal Article20190901Four triterpene barringotogenol C glycosidesnamely; 3-<em>O</em>-[α-L-rhamnopyranosyl (1-4)β-D-glucopyranosyl(1-4)-β-D-glucouronopyranoside]-21-<em>O</em>-α-L-rhamnopyranoside <strong>barringotogenolC(I)</strong>, 3-<em>O</em>-[α-L-rhamnopyranosyl(1-4) β-glucopyranosyl(1-4)β-D-galactopyranosyl]21-O-α-L- rhamnopyranoside<strong>barringotogenolC(II)</strong>,3-<em>O</em>-[β-D-glucopyranosyl(1-4) β-D-glucopyranosyl (1-4) β-D glucuronopyranoside] 21-<em>O</em>-α-L-rhamnopyranoside<strong>barringotogenolC(III)</strong>,3-<em>O</em>-[β-D-glucopyranosyl(1-6)β-D-glucopyranosyl]21-<em>O</em>-β-D-glucopyranoside<strong>barringotogenol C(IV)</strong>,Were isolated for the first time from the aerial part of <em>Medicago sativa L.</em>All compounds (<strong>I-IV</strong>) were identified by combination of spectroscopic methods (<strong>ID & 2DNMR</strong>) and compared with literature data.The extracts (Methanol & Dichloromethane) & isolated pure compounds were evaluated <em>in vitro</em> for their antimicrobial activity against <em>Streptococcus pneumoniae</em> (<strong>RCMB010010</strong>), <em>Staphylococcus aureus</em> (<strong>RCMB010028</strong>) (Gram+vestrain bacteria), <em>Pseudomonas aeuroginosa</em> (<strong>RCMB010043</strong>), <em>Escherichia coli</em> (<strong>RCMB010052</strong>) (Gram -vestrainbacteria) and Antifungal activity against <em>Aspergillus fumigatus</em> (<strong>RCMB02568</strong>) and <em>Candida albicans</em> (<strong>RCMB05036</strong>). Furthermore, theywere evaluated for their <em>in vitro</em> Anti-diabetic activity using α-Amylase inhibition method.https://ajps.journals.ekb.eg/article_46628_00aaddbe2ba1f4d06cce4b44c9c0c504.pdfAl-Azhar University, Faculty of PharmacyAl-Azhar Journal of Pharmaceutical Sciences1110-164457120180301DETECTION AND IDENTIFICATION OF CLOSTRIDIUM PERFRINGENS IN SOME LOCALLY ORAL PHARMACEUTICAL SAMPLES891034663010.21608/ajps.2018.46630ENSherinSharafMicrobiology Department-National Organization of Drug Control and Research (NODCAR), Giza, EgyptJournal Article20190901Contamination of oral pharmaceuticals with microorganisms may lead to deleterious effects on the therapeutic properties of the drug, and may potentially cause injuries to intended recipients. Thirty contaminated samples were detected representing 2% out of 1500 total collected samples. Out of 30 contaminated samples, only six samples (20%) revealed the presence of <em>Clostridium</em><em> perfringens</em>. The biofilm assay was performed using the Microtiter Plate Method (MTP) for the six of the detected <em>C. perfringens</em> were three isolates biofilm forming. PCR multiplex was done for <em>C. perfringens </em>showed the presence of alpha toxin gene among all species. In spite of the current recommendation for a conventional detection method for <em>Closterium </em>spp., the application of multiplex PCR showed to be more, effective and able to detect <em>Clostridium</em> toxins genes.https://ajps.journals.ekb.eg/article_46630_3be784418654d7d4d004d06af85fde05.pdfAl-Azhar University, Faculty of PharmacyAl-Azhar Journal of Pharmaceutical Sciences1110-164457120180301POTENTIOMETRIC DETERMINATION OF GLYCOPYRRONIUM BROMIDE IN BINARY MIXTURE WITH INDACATEROL MALEATE USING POLYVINYL CHLORIDE (PVC) MEMBRANE, COATED GRAPHITE AND CARBON PASTE SENSORS1041214663210.21608/ajps.2018.46632ENMohammedEl DosokyPharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Al-Azhar University, 11751 Nasr City, Cairo, EgyptJournal Article20190901A novel approach was conducted in this article for the determination of glycopyrronium bromide (GLY) in binary mixture with indacaterol maleate using ion selective electrodes for its selective determination in pure form and pharmaceutical formulation. Four ion selective electrode sensors were designed for determination of glycopyrronium bromide namely; glycopyrronium bromide-polyvinyl chloride (GLY-PVC) membrane, GLY-Coated graphite sensor and GLY-modified carbon paste sensors. The sensors are based on the ion association complexes of GLY cation with sodium tetra phenyl borate (GLY-TPB) and phosphomolybidic acid (GLY-PMA) counter anions as ion exchange sites using dioctylphthalate (DOP) as plasticizer. Validation of the method shows suitability of the proposed sensors for use in the quality control assessment of GLY and for its routine analysis. The results obtained were statistically compared to reported method and there was no significant difference between the proposed methods and the reported method regarding the accuracy and precision. The method was validated according to International Conference on Harmonization (ICH) guidelines and the results were satisfactory.https://ajps.journals.ekb.eg/article_46632_4e315d129fb125077bf05343282a6bad.pdf