THE CORRELATION OF THE BRCA-IRIS GENE EXPRESSION AND BIOMARKER GENES IN BREAST CANCER PATIENTS: CORRELATION WITH TUMOR PROGRESSION AND RESPONSE TO TREATMENT

Document Type : Original Article

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Department of Cancer Biology, Virology and Immunology Unit, National Cancer Institute, Cairo University, Egypt.

Abstract

Background: Triple-negative breast cancer (TNBC) is an aggressive phenotype with bad prognosis and poor survival. The prognostic and predictive values of BRCA1-Iris, octamer-binding transcription factor 4 (oct 4), cyclin D1andsurvivin were assessed in TNBC patients compared with N-TNBC patients.
 Method: RNA expression levels of BRCA1-IRIS, Oct4, Cyclin D1 and Survivin gene were tested in for 100 breast cancer patients by qRT-PCR.
Result: overexpression ofBRCA1-IRIS RNA was found in 49 % of breast cancer patients [(35(71 %) of TNBC cases vs 14(28.6% of N-TNBC] (p < 0.001). There was significantly correlated between the BRCA1-IRIS and tumor stage, ER and PR (p=0.036, p < 0.001 and p=0.006; respectively) in breast cancer patients. In TNBC or N-TNBC, no statistically significance between BRCA1-IRIS and any clinicopathological features of patients. There was significant association between BRCA1-IRIS positive and the expression of oct4, cyclin D1 and survivin gene in TNBC patients (p < 0.001, p=0.001 and p=0.002; respectively). There was a strong significant correlation between the expression of oct4 and the BRCA1-IRIS negative (p < 0.001) in TNBC group. No significant association between BRCA1-IRISpositive in N-TNBC and any of the gene expression. BRCA1-IRISnegative was correlated significantly with oct4 and cyclin D1expression (p=0.001each).No significant correlation between the expression RNA level of oct4, cyclin D1 and survivin gene and the expression RNA level of BRCA1-IRIS except lymphnode with survivin gene expression (p=0.05). No significant relation between the expression level of oct4, cyclin D1 and survivin gene and the relevant clinicopathological features with or without BRCA1-IRIS expression. Expression level of survivin and cyclin D1 was significantly correlation with the response to treatment (p=0.001 and p < 0.001). No significant different between overall survival and BRCA1-IRIS expression (p=0.291 long rang). However, disease-free survival was increased in BRCA1-IRIS positive cases (p = 0.052 long rang)

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