EFFECT OF AGNO3 NANOPARTICLES ON STERILIZATION AND ALKALOID PRODUCTION OF CATHERENTHUS ROSEUS TISSUE BY

Document Type : Original Article

Author

Genetic Engineering and Biotechnology Research Institute (GEBRI), Sadat City University, Sadat City, P.O. Box 79/22857, Egypt

Abstract

Bacterial contamination is a serious problem in plant tissue culture procedures.
An experiment has been conducted to evaluate the potential of nanosilver (NS) to remove bacterial contaminants from Catherenthus shoot tips and single nodes.
The experiments were performed as a totally randomized design in a faculty with four replications and each replicate with ten explants. Treatments involved NS in two steps (before and after surface sterilization along with control) at three concentrations (25, 50 and 100 mg 1-1) at three times (30, 45 and 60 minutes). Plants were grown on MS medium supplemented with 5 mg l-1of Kinetin (KIN)  and 0.1 mgl-1 of naphthaline acetic acid (NAA). The results showed that the use of 100 mg l-1 NS solution after sterilization of the surface resulted in the highest percentage (89%) of disinfected explants. Nano silver solution did not affect the measured characters. According to the data obtained in the experience, it was concluded that NS had a good view to eliminate bacterial diseases in plant tissue culture procedures. Further research on other cultural species requires that in the tissue cultures of other cultures, to enhance the effectiveness of the NS in order to eliminate bacterial diseases.

Keywords

Main Subjects

Files

Share

How to cite

Statistics