The effect of agitation speed and incubation period on the production of mannanase enzyme by Bacillus velezensis NRC-1 using bench-scale bioreactor at 45ºC was investigated. Results revealed that the increase in agitation speed from 200 to 800 rpm resulted in a significant increase in mannanase production to 21.04 U/mL. The increase in agitation speed affected dissolved oxygen (DO) concentration which in turn affected cell growth and mannanase production. The maximum mannanase production in the bioreactor was attained after 72 h of incubation at 45 ºC. Mannanase activity in the bioreactor (21.04 U/mL) while much higher than that obtained from the shake flask fermentation (15.6 U/mL) also the incubation period decreased from 7 days in shake flask fermentation to 3 days in bioreactor.
Mazeed, T. (2012). OPTIMIZATION OF AGITATION AND INCUBATION PERIOD ON PRODUCTION OF MANNANASE BY BACILLUS VELEZENSIS NRC-1 USING BENCH-SCALE BIOREACTOR. Al-Azhar Journal of Pharmaceutical Sciences, 45(1), 265-273. doi: 10.21608/ajps.2012.7243
MLA
Tarek Mazeed. "OPTIMIZATION OF AGITATION AND INCUBATION PERIOD ON PRODUCTION OF MANNANASE BY BACILLUS VELEZENSIS NRC-1 USING BENCH-SCALE BIOREACTOR". Al-Azhar Journal of Pharmaceutical Sciences, 45, 1, 2012, 265-273. doi: 10.21608/ajps.2012.7243
HARVARD
Mazeed, T. (2012). 'OPTIMIZATION OF AGITATION AND INCUBATION PERIOD ON PRODUCTION OF MANNANASE BY BACILLUS VELEZENSIS NRC-1 USING BENCH-SCALE BIOREACTOR', Al-Azhar Journal of Pharmaceutical Sciences, 45(1), pp. 265-273. doi: 10.21608/ajps.2012.7243
VANCOUVER
Mazeed, T. OPTIMIZATION OF AGITATION AND INCUBATION PERIOD ON PRODUCTION OF MANNANASE BY BACILLUS VELEZENSIS NRC-1 USING BENCH-SCALE BIOREACTOR. Al-Azhar Journal of Pharmaceutical Sciences, 2012; 45(1): 265-273. doi: 10.21608/ajps.2012.7243
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